Generuler Biotinylated DNA Ladder 生物素标记DNA分子(旧货号600-0008)
2018-10-25
PACKAGE INSERTKPL GeneRuler™ Biotinylated DNA LadderCatalog No.Size5960-0029(600-0008)100 μLwww.seracare.comL-1003848-01June 2017508.244.6400•800.676.1881 Toll Free•508.634.3334 FaxPage 1of 1DESCRIPTIONThe KPL GeneRuler™Biotinylated DNA Ladderprovides aconvenient pre-labeled molecular weight marker ideal for useon Southern blots. The digest contains a mixture of labeled andunlabeled DNA, allowing visualization either through ethidiumbromide staining or enzymatic detection as desired. Labeledand unlabeled DNA fragments migrate uniformly.CONTENTThe ladder is supplied in 10 mM Tris-HCl1.0 mM EDTA.Sufficient material is supplied to produce 20-50 marker lanes.STORAGESTABILITYStore at -20°C. Stable for a minimum of one year from date ofreceipt when stored at -20°C. The ladder will remain stablethrough at least 20 freeze-thaw cycles.USEDepurination is sometimes recommended for completenicking of Genomic DNA. This will result in loss of somelabeled fragments from the ladder. Therefore, use of moreladder in procedures where depurination is required isrecommended.Gel Loading:1.Mix 2 μL (if no depurination is required) to 5 μL (ifdepurination is required) of KPL GeneRuler Biotinylated DNALadder with the appropriate volume of gel loading buffer.2.Load ladder onto an agarose gel and perform gel electrophoresisfollowing standard procedures.Following
