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Formamide Hybridization Buffer 甲酰胺杂交缓冲液(旧货号50-86-10)

2018-10-25    


DESCRIPTIONKPL Formamide Hybridization Buffer is a formamide-basedsolution for use in the pre-hybridization and hybridization ofbiotinylated nucleic acid probes to nucleic acids fixed onmembranes. As a pre-hybridization solution, the buffer blockssites on the membrane and prevents nonspecific binding ofbiotinylated probe. As a hybridization solution, the bufferfacilitates binding of biotinylated probes to homologous RNAor DNAon a membrane.FORMSTORAGESTABILITYKPL Formamide Hybridization Buffer consists of 2 x 120 mLStoreat 2-8°C. Stable for a minimum of one year from date ofreceipt when stored at 2-8°C. KPL Formamide HybridizationBuffer will precipitate upon storage at cold temperatures. Forbest results, warm buffer to 37°C until all components are insolution, and aliquot into DNaseRNase free tubes.CONTENTKPL Formamide Hybridization Buffer is a formamide-basedsolution prepared with DNaseRNase free reagents.APPLICATIONSKPL Formamide Hybridization Buffer may be used for detection ofbiotinylated probes on membranes in procedures such asNorthern and Southern Blotting, and dot blots.USEHybridization temperature should be optimized between42°C-68°C depending on the melting temperature of theprobe. Prior to use, sheared and denatured KPL Herring SpermDNA must be added to a finalconcentration of 100 μg per mL.Pre-hybridization:1.Warm at least 0.1 ml of Formamide Hybridization Bufferper cm2of membrane. A minimum volume of 3mL isrequired when using a hybridization bottle (4 cm diameterx 14 cm long). If using less than 3 mL, a hybridizationbag is recommended.2.Add sheared, denatured herring sperm DNA to the buffer to a final concentration of 100 μg per mL天博